{"title":"一种抑制粗神经孢子虫腺苷酸环化酶突变的抑制突变","authors":"Tadako Murayama, Yasuyuki Fujisawa, Yoko Okano","doi":"10.1006/emyc.1995.1039","DOIUrl":null,"url":null,"abstract":"<div><p>Murayama, T., Fujisawa, Y., and Okano, Y. 1995. A suppressor mutation which suppresses adenylyl cyclase mutations in <em>Neurospora crassa. Experimental Mycology</em> 19, 320-323. A spontaneous suppressor mutant, <em>hah</em>, which suppressed the colonial growth of adenylyl cyclase mutants (<em>cr-1</em>) was isolated. The morphology of <em>cr-1 hah</em> was filamentous, but slightly different from that of wild type on solid medium. The <em>hah</em> strain formed many high aerial hyphae, but did not form any conidia. The expression levels of an adenylyl cyclase gene, <em>nac</em>, in both <em>hah</em> and <em>cr-1 hah</em> were much higher than those in wild type or in <em>cr-1</em>. The level of cAMP in <em>cr-1</em> was very low but returned to close to the wild-type level in the <em>cr-1 hah</em> suppressed strain, whereas that in the strain carrying <em>hah</em> alone was similar to or a little higher than that in wild type. The <em>hah</em> gene was located 13.3 map units from <em>inl</em> on the right arm of the linkage group V. The <em>hah</em> mutation was recessive and allele specificity of <em>hah</em> for the suppression of <em>cr-1</em> mutations was weak.</p></div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"19 4","pages":"Pages 320-323"},"PeriodicalIF":0.0000,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1995.1039","citationCount":"5","resultStr":"{\"title\":\"A Suppressor Mutation Which Suppresses Adenylyl Cyclase Mutations in Neurospora crassa\",\"authors\":\"Tadako Murayama, Yasuyuki Fujisawa, Yoko Okano\",\"doi\":\"10.1006/emyc.1995.1039\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Murayama, T., Fujisawa, Y., and Okano, Y. 1995. A suppressor mutation which suppresses adenylyl cyclase mutations in <em>Neurospora crassa. Experimental Mycology</em> 19, 320-323. A spontaneous suppressor mutant, <em>hah</em>, which suppressed the colonial growth of adenylyl cyclase mutants (<em>cr-1</em>) was isolated. The morphology of <em>cr-1 hah</em> was filamentous, but slightly different from that of wild type on solid medium. The <em>hah</em> strain formed many high aerial hyphae, but did not form any conidia. The expression levels of an adenylyl cyclase gene, <em>nac</em>, in both <em>hah</em> and <em>cr-1 hah</em> were much higher than those in wild type or in <em>cr-1</em>. The level of cAMP in <em>cr-1</em> was very low but returned to close to the wild-type level in the <em>cr-1 hah</em> suppressed strain, whereas that in the strain carrying <em>hah</em> alone was similar to or a little higher than that in wild type. The <em>hah</em> gene was located 13.3 map units from <em>inl</em> on the right arm of the linkage group V. The <em>hah</em> mutation was recessive and allele specificity of <em>hah</em> for the suppression of <em>cr-1</em> mutations was weak.</p></div>\",\"PeriodicalId\":12110,\"journal\":{\"name\":\"Experimental Mycology\",\"volume\":\"19 4\",\"pages\":\"Pages 320-323\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1006/emyc.1995.1039\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0147597585710390\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Mycology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147597585710390","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
摘要
Murayama, T., Fujisawa, Y.和Okano, Y. 1995。一种在粗神经孢子虫中抑制腺苷酸环化酶突变的抑制突变。真菌学通报,19(2):349 - 349。分离到一个抑制腺苷酸环化酶突变体(cr-1)群体生长的自发抑制突变体hah。cr- 1hah的形态呈丝状,但与野生型在固体培养基上略有不同。hah菌株形成了许多高空菌丝,但没有形成任何分生孢子。腺苷酸环化酶基因nac在ha和cr-1 ha中的表达量明显高于野生型和cr-1。抑制菌株的cAMP在cr-1中含量很低,但恢复到接近野生型水平,而单独携带hah的菌株的cAMP与野生型相似或略高于野生型。hah基因位于连锁组v右臂距in1的13.3个图谱单位处,hah突变为隐性突变,其抑制cr-1突变的等位基因特异性较弱。
A Suppressor Mutation Which Suppresses Adenylyl Cyclase Mutations in Neurospora crassa
Murayama, T., Fujisawa, Y., and Okano, Y. 1995. A suppressor mutation which suppresses adenylyl cyclase mutations in Neurospora crassa. Experimental Mycology 19, 320-323. A spontaneous suppressor mutant, hah, which suppressed the colonial growth of adenylyl cyclase mutants (cr-1) was isolated. The morphology of cr-1 hah was filamentous, but slightly different from that of wild type on solid medium. The hah strain formed many high aerial hyphae, but did not form any conidia. The expression levels of an adenylyl cyclase gene, nac, in both hah and cr-1 hah were much higher than those in wild type or in cr-1. The level of cAMP in cr-1 was very low but returned to close to the wild-type level in the cr-1 hah suppressed strain, whereas that in the strain carrying hah alone was similar to or a little higher than that in wild type. The hah gene was located 13.3 map units from inl on the right arm of the linkage group V. The hah mutation was recessive and allele specificity of hah for the suppression of cr-1 mutations was weak.