臭氧对伴有周围血管疾病的2型糖尿病患者血红蛋白氧亲和力的影响:体外研究

Diabete & metabolisme Pub Date : 1995-10-01
L Coppola, R Giunta, G Verrazzo, C Luongo, A Sammartino, C Vicario, D Giugliano
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引用次数: 0

摘要

臭氧在周围血管疾病(PVD)治疗中的应用越来越多。本研究的目的是评估臭氧对2型糖尿病合并PVD患者血红蛋白氧亲和力的影响。研究对象为20例伴有PVD的糖尿病患者(Fontaine的临床分期为II-IV期)和20例非糖尿病健康人。在两组中,等量的血液用氧-臭氧(O2-O3)的混合物进行臭氧处理,达到每毫升底物6.5、13、26和78微克O3的终浓度。在基线时,糖尿病患者的血红蛋白氧亲和力值明显低于对照组,但血浆游离血红蛋白和丙二醛(MDA)水平高于对照组。在糖尿病患者和对照组中,血液暴露于臭氧以“全有或全无”的方式降低了血红蛋白对氧的亲和力,而红细胞中2-3个二磷酸甘油脂的浓度没有改变。游离血红蛋白和丙二醛浓度在血液臭氧化后均表现出明显的剂量依赖性增加。因此,臭氧导致正常受试者和2型糖尿病合并PVD患者血红蛋白氧卸载显著增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of ozone on haemoglobin oxygen affinity in type-2 diabetic patients with peripheral vascular disease: in vitro studies.

The use of ozone in the treatment of peripheral vascular disease (PVD) is increasing. The purpose of this study was to evaluate the effect of ozone on haemoglobin oxygen affinity in Type-2 diabetic patients with PVD. Twenty diabetic patients presenting with PVD (Clinical stage II-IV according to Fontaine) and 20 non-diabetic healthy matched subjects were studied. In both groups, aliquots of blood were ozonised with mixtures of oxygen-ozone (O2-O3) to reach end-concentrations of 6.5, 13, 26 and 78 micrograms O3 per ml of substrate. At baseline, diabetic patients presented significantly lower haemoglobin oxygen affinity values but higher plasma levels of free haemoglobin and malonyldialdehyde (MDA) than controls. In both diabetic patients and controls, exposure of blood to ozone reduced haemoglobin oxygen affinity in an "all-or-none" fashion, without changing 2-3, diphosphoglycerate concentrations in erythrocytes. Both free haemoglobin and MDA concentrations showed significant, dose-dependent increases after blood ozonisation. Thus, ozone caused a significant increase in oxygen unloading of haemoglobin in both normal subjects and Type-2 diabetic patients with PVD.

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