用理化方法纯化PVYo及其可溶性抗原。

Preparative biochemistry Pub Date : 1993-02-01 DOI:10.1080/10826069308544556

A Polson

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引用次数: 0

摘要

在病毒提取转子中，将感染后切碎的植物组织离心纯化马铃薯Yo病毒。由于烟草花叶病毒(TMV)对初播材料的严重污染，对该病毒进行了分离，并在鸡中获得了抗体。利用鸡抗TMV抗体(IgY)，在PVYo在烟草植株上繁殖之前，将这种外来病毒从PVYo的原始种子材料中去除。

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Purification of PVYo and its soluble antigen by physico chemical means.

Potato virus Yo was purified by centrifugation of infected and minced plant tissue in the virus extraction rotor. As the initial seeding material was heavily contaminated with tobacco mosaic virus (TMV) this virus was isolated and antibody was elicited in chickens. The chicken antibody (IgY) against TMV was used for removing this extraneous virus from the original PVYo seeding material prior to propagating PVYo in tobacco plants, CV Glutinosa.

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Preparative biochemistry

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