药品和化妆品保鲜效果快速检测方法的研究。

P Connolly, S F Bloomfield, S P Denyer
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引用次数: 35

摘要

评价了阻抗法、直接荧光法(DEFT-MEM)和ATP生物发光法(ATP- b) 3种快速微生物学方法在药品和化妆品保鲜效果检测(PET)中的适用性。对于未经处理的金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌悬浮液,三种方法均获得了快速方法反应与总菌落计数的良好相关性,但对于黑曲霉,只有阻抗。氯己定处理的葡萄球菌混悬液。阻抗法对金黄色葡萄球菌和白色念珠菌的杀伤效果较好,但ATP-B法和DEFT-MEM法的杀伤效果低估了1 ~ 6个对数。从本研究的结果可以得出结论,阻抗为PET的菌落计数方法提供了一种替代方法,但在目前的方法发展水平下,DEFT-MEM和ATP-B都不能被认为是令人满意的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A study of the use of rapid methods for preservative efficacy testing of pharmaceuticals and cosmetics.

Three rapid microbiological methods, impedance, the direct epifluorescence technique (DEFT-MEM) and ATP bioluminescence (ATP-B) were evaluated for their applicability to preservative efficacy testing (PET) of pharmaceuticals and cosmetics. A good correlation between rapid method response and total colony counts was obtained for untreated suspensions of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans with all three methods but, for Aspergillus niger, with impedance only. For chlorhexidine-treated suspensions of Staph. Aureus and C. albicans, a good dose-response curve was obtained with impedance, but ATP-B and DEFT-MEM methods underestimated the kill by the order of 1-6 logs. From the results of this study it is concluded that impedance offers an alternative method to colony counting methods for PET but, at their present level of method development, neither DEFT-MEM nor ATP-B can be considered as satisfactory.

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