检验生牛肉产品是否含有产生Vero细胞毒素的大肠杆菌,特别是血清群为O157的产品。

G A Willshaw, H R Smith, D Roberts, J Thirlwell, T Cheasty, B Rowe
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引用次数: 73

摘要

310份生牛肉产品样品中有54份(17%)含有产Vero细胞毒素(VT)的大肠杆菌(VTEC)。从选择的阳性样本中详细检查的VTEC菌株属于几个O血清群,其中一些与人类腹泻或溶血性尿毒综合征有关。有些菌株具有可能对人产生毒力的特性。采用VT探针试验和集落免疫印迹法结合市售抗O157血清检测,所有食品样品均不含产生VT的大肠杆菌O157。免疫印迹定量技术表明,O157 VTEC可从人工接种的肉类样品中以低于每克1个生物的水平回收。其中5份食品样本携带的大肠杆菌O157菌株不产生VT,并且在其他特性上与O157 VTEC不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Examination of raw beef products for the presence of Vero cytotoxin producing Escherichia coli, particularly those of serogroup O157.

Fifty-four of 310 (17%) samples of raw beef products contained Vero cytotoxin (VT)-producing Escherichia coli (VTEC) detected by DNA probes for the VT genes. VTEC strains examined in detail from a selection of the positive samples belonged to several O serogroups, some of which have been associated with human diarrhoea or haemolytic uraemic syndrome. Some of the strains possessed properties that may contribute to virulence in man. None of the food samples contained VT-producing E. coli O157 when tested by a combination of VT probe tests and colony immunoblotting with commercially available anti-O157 serum. Quantification of the immunoblotting technique indicated that O157 VTEC could be recovered from artificially-inoculated meat samples at a level of less than one organism per gram. Five of the food samples carried E. coli O157 strains that did not produce VT and differed in other properties from O157 VTEC.

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