{"title":"一种从牛精浆中纯化精浆蛋白的简单有效方法。","authors":"D B Kameswari, K S Prasad","doi":"10.1080/10826069408010079","DOIUrl":null,"url":null,"abstract":"<p><p>A method involving direct affinity chromatography of undialysed bovine seminal plasma on a calmodulin-agarose column was developed for the purification of seminalplasmin. Seminalplasmin thus obtained was further purified from any contaminants left by ion-exchange chromatography on a short CM-Sepharose column. The method gave an excellent yield of seminalplasmin (0.7 mg per ml seminal plasma) that showed 100% inhibition of bacterial growth at a concentration of 10 micrograms/ml. The purified seminalplasmin was found to be homogeneous as tested by HPLC on a reverse-phase column and SDS-PAGE.</p>","PeriodicalId":20391,"journal":{"name":"Preparative biochemistry","volume":"24 1","pages":"15-24"},"PeriodicalIF":0.0000,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10826069408010079","citationCount":"1","resultStr":"{\"title\":\"A simple and efficient method for the purification of seminal plasmin, an antimicrobial protein from bovine seminal plasma.\",\"authors\":\"D B Kameswari, K S Prasad\",\"doi\":\"10.1080/10826069408010079\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A method involving direct affinity chromatography of undialysed bovine seminal plasma on a calmodulin-agarose column was developed for the purification of seminalplasmin. Seminalplasmin thus obtained was further purified from any contaminants left by ion-exchange chromatography on a short CM-Sepharose column. The method gave an excellent yield of seminalplasmin (0.7 mg per ml seminal plasma) that showed 100% inhibition of bacterial growth at a concentration of 10 micrograms/ml. The purified seminalplasmin was found to be homogeneous as tested by HPLC on a reverse-phase column and SDS-PAGE.</p>\",\"PeriodicalId\":20391,\"journal\":{\"name\":\"Preparative biochemistry\",\"volume\":\"24 1\",\"pages\":\"15-24\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/10826069408010079\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Preparative biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/10826069408010079\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Preparative biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/10826069408010079","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A simple and efficient method for the purification of seminal plasmin, an antimicrobial protein from bovine seminal plasma.
A method involving direct affinity chromatography of undialysed bovine seminal plasma on a calmodulin-agarose column was developed for the purification of seminalplasmin. Seminalplasmin thus obtained was further purified from any contaminants left by ion-exchange chromatography on a short CM-Sepharose column. The method gave an excellent yield of seminalplasmin (0.7 mg per ml seminal plasma) that showed 100% inhibition of bacterial growth at a concentration of 10 micrograms/ml. The purified seminalplasmin was found to be homogeneous as tested by HPLC on a reverse-phase column and SDS-PAGE.
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