tcr - α， β和tcr - γ， δ /CD3复合物的生物合成。

Immunodeficiency Pub Date : 1993-01-01

S Bauguil-Caspar, J Arnaud, J Kuhlmann, C Geisler, B Rubin

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引用次数: 0

摘要

用WT31单抗(Tcr- α， β)或Tcr- δ 1单抗(Tcr- γ， δ)检测转染J76- α cDNA或J76 Tcr- α突变cDNA (J79)的Jurkat J76克隆、LYON L12.37克隆和L12.37细胞Tcr/CD3复合物的膜表达。转染J76 tcr - α cDNA的LYON细胞具有胞内tcr - γ、δ链，转染J79 tcr - α cDNA的LYON细胞具有胞内tcr - α (M)、β链。

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Biosynthesis of Tcr-alpha, beta and Tcr-gamma, delta/CD3 complexes.

Jurkat J76 clone, LYON L12.37 clone and L12.37 cells transfected with J76-alpha cDNA or J76 Tcr-alpha mutated cDNA (J79) were analysed for membrane expression of Tcr/CD3 complex using WT31 mAb (Tcr-alpha, beta) or Tcr-delta 1 mAb (Tcr-gamma, delta): LYON cells express V beta 9 bearing Tcr-beta chains. J76 Tcr-alpha cDNA transfected LYON cells have intracellular Tcr-gamma, delta chains and J79 Tcr-alpha cDNA transfected LYON cells have intracellular Tcr-alpha (M), beta chains.

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Immunodeficiency

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