猕猴肝色素细胞黑素体atp酶活性的研究。

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry Pub Date : 1994-08-01 DOI:10.1016/0305-0491(94)90105-8

G. Sichel , C. Corsaro , E. Cassiani , C. Magnani , L. Bolognani

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引用次数: 1

摘要

荧光光度法表明，斑蛙肝色素细胞中的黑素体具有内源性ATP和ATP酶活性。在pH 7.0时，atp酶的Km值为0.42 × 10−8 mol/l。抗霉素和沃巴因对atp酶的抑制无效。在过量ADP和Pi存在的情况下，观察到ATP的合成。

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ATPase activity of melanosomes in liver pigment cells of Rana esculenta L.

Luminometric methods show that melanosomes in liver pigment cells of Rana esculenta L. have endogenous ATP and ATPase activity. The K_m value of ATPase is 0.42 × 10⁻⁸ mol/l at pH 7.0. Inhibition of ATPase by antimycin and by ouabain is not effective. In the presence of an excess of ADP and Pi, ATP synthesis was observed.

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Comparative Biochemistry and Physiology Part B: Comparative Biochemistry

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