雄性豚鼠骨盆脏器神经元的化学编码:逆行运输和免疫组织化学的研究。

The Histochemical Journal Pub Date : 1994-03-01
D Dhami, B S Mitchell
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引用次数: 0

摘要

使用Fast Blue染料进行的逆行运输研究表明,雄性豚鼠的输精管、精囊、前列腺和直肠(而不是膀胱)至少部分受骨盆前大神经节支配。在输精管、精囊和前列腺的神经支配来源于同侧和对侧神经节。除了逆行研究外,染色填充的神经元用免疫组织化学方法分析神经元标记物和与特异性识别的神经元投射的关联。神经节上投射到断管的神经元分别含有单独的酪氨酸羟化酶、酪氨酸羟化酶和神经肽Y、单独的神经肽Y、神经肽Y和血管活性肠肽或单独的血管活性肠肽。这些神经元与三种类型的神经元投射有关,P-物质,亮氨酸-脑啡肽-和蛋氨酸-脑啡肽免疫反应。除精囊特异性神经元没有表现出血管活性肠肽免疫反应性外,投射到精囊的神经元与供应输精管的神经元相似。供给前列腺的神经元对酪氨酸羟化酶或神经肽Y均有免疫反应;这些神经元很少与三种类型的静脉曲张神经元投射相关。在一只动物中,投射到直肠的神经元含有神经肽Y,仅与蛋氨酸-脑啡肽免疫反应性神经元投射有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chemical coding of neurons projecting to pelvic viscera in the male guinea pig: a study by retrograde transport and immunohistochemistry.

Retrograde transport studies using Fast Blue dye demonstrated that the ductus deferens, seminal vesicle, prostate and rectum, but not the urinary bladder of the male guinea pig are at least in part innervated by the anterior major pelvic ganglion. In the ductus deferens, seminal vesicle and prostate innervation is derived from ipsilateral and contralateral ganglia. In addition to retrograde studies, dye-filled neurons were analysed immunohistochemically for neuronal markers and associations with specifically identified neuronal projections. Neurons of the ganglion projecting to the ductus deferens either contained tyrosine hydroxylase alone, tyrosine hydroxylase and neuropeptide Y, neuropeptide Y alone, neuropeptide Y and vasoactive intestinal peptide, or vasoactive intestinal peptide alone. These neurons were associated with three classes of neuronal projections, substance P-, leucine-enkephalin-, and methionine-enkephalin-immunoreactive. Neurons projecting to the seminal vesicles were similar to the neurons supplying the ductus deferens, except none of the seminal vesicle-specific neurons exhibited vasoactive intestinal peptide immunoreactivity. Neurons supplying the prostate were immunoreactive for either tyrosine hydroxylase or neuropeptide Y; these neurons were infrequently associated with the three classes of varicose neuronal projections. Neurons projecting to the rectum contained neuropeptide Y and were only associated with methionine-enkephalin immunoreactive neuronal projections in one animal.

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