H. Fujinami , K. Komabayashi , T. Izawa , K. Suda , M. Tsuboi
{"title":"大鼠腮腺短期热暴露期间毒蕈碱受体和毒蕈碱刺激诱导的Ca2+动员的体内适应性调节","authors":"H. Fujinami , K. Komabayashi , T. Izawa , K. Suda , M. Tsuboi","doi":"10.1016/0742-8413(93)90085-Y","DOIUrl":null,"url":null,"abstract":"<div><p>1. Adaptation of muscarinic receptors (MR)—muscarinic stimulation—induced intracellular Ca<sup>2+</sup> mobilization during short-heat exposure (33°C).</p><p>2. Heat-exposure for 48 hr decreased the carbachol (CCh)-stimulated cytosolic C<sup>2+</sup> concentration increase.</p><p>3. The number of MR on cell surface increased transiently at 24 hr with a subsequent decrease at 48 hr.</p><p>4. CCh-stimulated inositol trisphosphate (IP<sub>3</sub>) formation decreased at 48 hr.</p><p>5. In saponin-permeabilized cells, 1,4,5-IP<sub>3</sub>-induced <sup>45</sup>Ca<sup>2+</sup> release decreased at 24 hr.</p><p>6. The data suggest that the adaptation for increased muscarinic stimulation occurs at IP<sub>3</sub> generating sites as well as at intracellular IP<sub>3</sub> receptor sites during heat exposure.</p></div>","PeriodicalId":72650,"journal":{"name":"Comparative biochemistry and physiology. C: Comparative pharmacology","volume":"105 3","pages":"Pages 451-457"},"PeriodicalIF":0.0000,"publicationDate":"1993-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0742-8413(93)90085-Y","citationCount":"3","resultStr":"{\"title\":\"In vivo adaptative regulation of muscarinic receptors and muscarinic stimulation-induced Ca2+ mobilization during short-term heat exposure in rat parotid glands\",\"authors\":\"H. Fujinami , K. Komabayashi , T. Izawa , K. Suda , M. Tsuboi\",\"doi\":\"10.1016/0742-8413(93)90085-Y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>1. Adaptation of muscarinic receptors (MR)—muscarinic stimulation—induced intracellular Ca<sup>2+</sup> mobilization during short-heat exposure (33°C).</p><p>2. Heat-exposure for 48 hr decreased the carbachol (CCh)-stimulated cytosolic C<sup>2+</sup> concentration increase.</p><p>3. The number of MR on cell surface increased transiently at 24 hr with a subsequent decrease at 48 hr.</p><p>4. CCh-stimulated inositol trisphosphate (IP<sub>3</sub>) formation decreased at 48 hr.</p><p>5. In saponin-permeabilized cells, 1,4,5-IP<sub>3</sub>-induced <sup>45</sup>Ca<sup>2+</sup> release decreased at 24 hr.</p><p>6. The data suggest that the adaptation for increased muscarinic stimulation occurs at IP<sub>3</sub> generating sites as well as at intracellular IP<sub>3</sub> receptor sites during heat exposure.</p></div>\",\"PeriodicalId\":72650,\"journal\":{\"name\":\"Comparative biochemistry and physiology. C: Comparative pharmacology\",\"volume\":\"105 3\",\"pages\":\"Pages 451-457\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1993-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0742-8413(93)90085-Y\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative biochemistry and physiology. C: Comparative pharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/074284139390085Y\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative biochemistry and physiology. C: Comparative pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/074284139390085Y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In vivo adaptative regulation of muscarinic receptors and muscarinic stimulation-induced Ca2+ mobilization during short-term heat exposure in rat parotid glands
1. Adaptation of muscarinic receptors (MR)—muscarinic stimulation—induced intracellular Ca2+ mobilization during short-heat exposure (33°C).
2. Heat-exposure for 48 hr decreased the carbachol (CCh)-stimulated cytosolic C2+ concentration increase.
3. The number of MR on cell surface increased transiently at 24 hr with a subsequent decrease at 48 hr.
4. CCh-stimulated inositol trisphosphate (IP3) formation decreased at 48 hr.
5. In saponin-permeabilized cells, 1,4,5-IP3-induced 45Ca2+ release decreased at 24 hr.
6. The data suggest that the adaptation for increased muscarinic stimulation occurs at IP3 generating sites as well as at intracellular IP3 receptor sites during heat exposure.
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