Naofumi Kitabatake, Etsushiro Doi, Alka B. Trivedi
{"title":"利用几种动物细胞系对真菌毒素、柑桔毒素和赭曲霉毒素进行毒性评价","authors":"Naofumi Kitabatake, Etsushiro Doi, Alka B. Trivedi","doi":"10.1016/0742-8413(93)90082-V","DOIUrl":null,"url":null,"abstract":"<div><p>1. The cytotoxicities of the nephrotoxic mycotoxins, citrinin and ochratoxin A were assayed on HeLa, C3H/10T1/2, NIH/3T3, MDCK (canine kidney), and HeLa P3 cell lines, using the MTT colorimetric assay.</p><p>2. Citrinin was less toxic than ochratoxin A in all of the cell lines examined.</p><p>3. The MDCK cells were more susceptible to both citrinin and ochratoxin A, in comparison with other cell lines.</p><p>4. Dose-responses, as measured by activities of leucine aminopeptidase and alkaline phosphatase of MDCK cells, were less sensitive than MTT colorimetric assay, indicating that these enzymes were not specifically inhibited in MDCK cells.</p><p>5. The <span>ld</span><sub>50</sub> of both toxins, calculated at 72 hr of incubation, was in the same order as those reported from animal experiments using rats and mice.</p></div>","PeriodicalId":72650,"journal":{"name":"Comparative biochemistry and physiology. C: Comparative pharmacology","volume":"105 3","pages":"Pages 429-433"},"PeriodicalIF":0.0000,"publicationDate":"1993-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0742-8413(93)90082-V","citationCount":"26","resultStr":"{\"title\":\"Toxicity evaluation of the mycotoxins, citrinin and ochratoxin a, using several animal cell lines\",\"authors\":\"Naofumi Kitabatake, Etsushiro Doi, Alka B. Trivedi\",\"doi\":\"10.1016/0742-8413(93)90082-V\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>1. The cytotoxicities of the nephrotoxic mycotoxins, citrinin and ochratoxin A were assayed on HeLa, C3H/10T1/2, NIH/3T3, MDCK (canine kidney), and HeLa P3 cell lines, using the MTT colorimetric assay.</p><p>2. Citrinin was less toxic than ochratoxin A in all of the cell lines examined.</p><p>3. The MDCK cells were more susceptible to both citrinin and ochratoxin A, in comparison with other cell lines.</p><p>4. Dose-responses, as measured by activities of leucine aminopeptidase and alkaline phosphatase of MDCK cells, were less sensitive than MTT colorimetric assay, indicating that these enzymes were not specifically inhibited in MDCK cells.</p><p>5. The <span>ld</span><sub>50</sub> of both toxins, calculated at 72 hr of incubation, was in the same order as those reported from animal experiments using rats and mice.</p></div>\",\"PeriodicalId\":72650,\"journal\":{\"name\":\"Comparative biochemistry and physiology. C: Comparative pharmacology\",\"volume\":\"105 3\",\"pages\":\"Pages 429-433\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1993-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0742-8413(93)90082-V\",\"citationCount\":\"26\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative biochemistry and physiology. C: Comparative pharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/074284139390082V\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative biochemistry and physiology. C: Comparative pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/074284139390082V","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Toxicity evaluation of the mycotoxins, citrinin and ochratoxin a, using several animal cell lines
1. The cytotoxicities of the nephrotoxic mycotoxins, citrinin and ochratoxin A were assayed on HeLa, C3H/10T1/2, NIH/3T3, MDCK (canine kidney), and HeLa P3 cell lines, using the MTT colorimetric assay.
2. Citrinin was less toxic than ochratoxin A in all of the cell lines examined.
3. The MDCK cells were more susceptible to both citrinin and ochratoxin A, in comparison with other cell lines.
4. Dose-responses, as measured by activities of leucine aminopeptidase and alkaline phosphatase of MDCK cells, were less sensitive than MTT colorimetric assay, indicating that these enzymes were not specifically inhibited in MDCK cells.
5. The ld50 of both toxins, calculated at 72 hr of incubation, was in the same order as those reported from animal experiments using rats and mice.
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