一种针对互补肽的单克隆自身抗体识别间质胶原酶

ImmunoMethods Pub Date : 1994-10-01 DOI:10.1006/immu.1994.1052

de Souza Sandro José, Madaio Michael P., Neto Luis Juliano, Brentani Ricardo Renzo

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引用次数: 4

摘要

描述了一种针对互补肽TKKTLRT的单克隆自身抗体(mab 16)，该抗体是从胶原蛋白中胶原酶敏感位点推断出来的。通过ELISA和免疫沉淀实验，Mab 16可以识别间质胶原酶。此外，mab 16能够部分抑制角质形成细胞上清的胶原溶解活性。最后，可以使用mab 16/Sepharose柱对胶原酶进行免疫纯化。

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A Monoclonal Autoantibody against a Complementary Peptide Recognizes Interstitial Collagenase

A monoclonal autoantibody (mab 16) against the complementary peptide TKKTLRT, which was deduced from the collagenase-sensitive site in collagen, is described. Mab 16 recognized interstitial collagenase as visualized by ELISA and immunoprecipitation assays. Moreover, mab 16 was able to inhibit partially the collagenolytic activity of keratinocyte supernatant. Finally, it was possible to immunopurify collagenase using a mab 16/Sepharose column.

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ImmunoMethods

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