Jinxuan Liang , Zhiwei Liu , Yalin Fang , Xing Shen , Zhenlin Xu , Hongtao Lei , Xianhui Huang , Xiangmei Li
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Two kinds of lateral flow immunoassays based on multifunctional magnetic prussian blue nanoenzyme and colloidal gold for the detection of 38 β-agonists in swine urine and pork
Herein, novel multifunctional magnetic prussian blue nanoenzymes (MPBNs) and colloidal gold (CG) were synthesized and used to develop two kinds of lateral flow immunoassays (LFIAs) for the detection of 38 β-agonists. Since MPBNs has a unique three-in-one function of colorimetric magnetic catalytic activities, the signal intensity and coupling ratio are 2 and 8-fold higher than that of the CG. The cut-off values of the CG-LFIA and MPBNs-LFIA for swine urine and pork are 5/5 and 0.3/0.5 μg/kg, the limits of detection are 0.19/0.29 and 0.02/0.03 μg/kg, respectively. The sensitivity of MPBNs-LFIA is 10-fold higher than that of CG-LFIA, and up to 200-fold higher than that of the reported LFIAs. The recoveries of the LFIAs are 80.0%–116.7%, with coefficients of variation of 1.4%–14.3%. Our study proved that the MPBNs have more advantages than CG, and can offer a promising signal label for ultrasensitive immunoassay techniques.
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Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.
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