十二烷基硫酸钠-聚丙烯酰胺凝胶电泳及双向电泳分析蛇毒。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1994-01-01

T Marshall, K M Williams

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引用次数: 0

摘要

采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS- page)和高分辨率双向电泳(2-DE)分析了蛇毒的蛋白质组成，并将简化的Tris/甘氨酸/SDS (TGS)方法与市产的Tris/Tricine/SDS (TTS)凝胶体系进行了比较。SDS-PAGE提示种间差异和亲缘种间的相似性，但2-DE提示毒液的蛋白质组成高度复杂。TTS系统提高了2-DE的分辨率，但我们的简化TGS方法是银染色的最佳方法，与之前的报道相反，它将检测提高了100倍。

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Analysis of snake venoms by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional electrophoresis.

The protein composition of snake venom has been analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high resolution two-dimensional electrophoresis (2-DE) with comparison of our simplified Tris/glycine/SDS (TGS) method with a commercial Tris/Tricine/SDS (TTS) gel system. SDS-PAGE suggests differences between species and similarities between related species but 2-DE indicates that the protein composition of the venoms is highly complex. The TTS system improves resolution upon 2-DE but our simplified TGS method is optimal for silver staining which, contrary to previous reports, enhances detection 100-fold.

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Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society

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