分娩对人脐带血白细胞、白细胞亚群和造血祖细胞数量的影响。

Blood cells Pub Date : 1994-01-01
F T Lim, L van Winsen, R Willemze, H H Kanhai, J H Falkenburg
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引用次数: 0

摘要

人脐带血(UCB)可作为异基因骨髓移植中骨髓再生细胞的替代来源。UCB移植采收的质量和数量受到几个因素的影响。在这项研究中,我们分析了分娩,特别是分娩期间的应激对UCB中白细胞和白细胞亚群数量的影响。四组不同分娩类型的妇女被纳入研究,并从每组的UCB样本进行分析。健康成人的血液样本作为对照。UCB中白细胞的绝对数量高于外周血(PB)。第二产程延长后,UCB白细胞最高,这主要是由于粒细胞增多所致。UCB中T细胞的百分比低于PB,特别是在分娩时应激较高时。然而,在所有组中,每毫升UCB中T细胞的绝对浓度都高于成人PB。应激分娩中T细胞的差异主要是由于UCB中CD3+/CD4+细胞的相对减少。UCB中CD56+细胞群的相对频率和绝对浓度高于PB,这主要是由于CD2-/CD56+细胞的增加,特别是在应激分娩中。在半固体培养基培养中,CD34+细胞和造血祖细胞的绝对数量在UCB中较高,并且在产程延长的情况下增加。本研究表明,UCB移植的质量受到分娩过程的影响,特别是分娩过程中的压力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of delivery on numbers of leukocytes, leukocyte subpopulations, and hematopoietic progenitor cells in human umbilical cord blood.

Human umbilical cord blood (UCB) may be used as an alternative source of bone marrow repopulating cells in allogeneic bone marrow transplantation. The quality and quantity of UCB harvests for transplantation is affected by several factors. In this study we analyzed the influence of delivery, in particular stress during delivery, on the numbers of leukocytes and leukocyte subsets in UCB. Four groups of women with different types of deliveries were included in the study, and from each group samples of UCB were analyzed. Blood samples from healthy adults were used as control. In UCB there was a higher absolute number of leukocytes than in peripheral blood (PB). UCB leukocytes were highest after deliveries with a prolonged second stage of labor, which was mainly due to granulocytosis. The percentage of T cells in UCB was lower than in PB, in particular when stress during delivery was higher. In all groups, however, the absolute concentration of T cells per milliliter of UCB was higher than in adult PB. The differences in T cells in stressful deliveries were mainly due to a relative decrease in CD3+/CD4+ cells in UCB. The relative frequency and absolute concentration of the CD56+ cell population in UCB was higher than in PB, which was mostly due to an increase of CD2-/CD56+ cells, in particular in stressful deliveries. The absolute number of CD34+ cells as well as hematopoietic progenitor cells as determined in semisolid medium cultures was high in UCB and was increased in cases of prolonged secondary stage of labor. This study demonstrates that the quality of UCB transplants is influenced by the course of delivery, in particular by stress during delivery.

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