脂质体包封muramyl三肽-磷脂酰乙醇胺(L-MTP-PE)对正常猫血清肿瘤坏死因子- α和白细胞介素-6活性的诱导作用。

L E Fox, R R King, F Shi, I D Kurzman, E G MacEwen, P S Kubilis
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引用次数: 6

摘要

肿瘤坏死因子- α (TNF - α)和白细胞介素-6 (IL-6)是活化单核/巨噬细胞的产物,具有抗肿瘤活性。脂质体包裹的muramyl三肽-磷脂酰乙醇胺(L-MTP-PE)是一种有效的单核细胞/巨噬细胞激活剂。猫经静脉注射L-MTP-PE后的血清通过WEHI-164细胞毒性试验显示TNF - α活性,通过IL-6依赖性小鼠7TD1杂交瘤细胞增殖试验显示IL-6活性。血清TNF - α活性在L-MTP-PE给药后2小时达到峰值。与脂当量对照组相比,在2和3小时观察到显著差异(P < 0.05)。通过连续稀释rhTNF α单克隆抗体来中和血清TNF α活性。血清IL-6活性在给予L-MTP-PE后3小时达到峰值。与脂当量对照组相比,在2、3和4小时观察到显著差异(P < 0.05)。山羊抗IL-6多克隆抗体不能中和血清IL-6活性。静脉注射L-MTP-PE可诱导正常猫血清TNF α和IL-6活性,而非脂质等量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Induction of serum tumor necrosis factor-alpha and interleukin-6 activity by liposome-encapsulated muramyl tripeptide-phosphatidylethanolamine (L-MTP-PE) in normal cats.

Tumor necrosis factor-alpha (TNF alpha) and interleukin-6 (IL-6) are products of activated monocytes/macrophages with anti-tumor activity. Liposome-encapsulated muramyl tripeptide-phosphatidylethanolamine (L-MTP-PE) is a potent monocyte/macrophage activator. Sera from cats after intravenous L-MTP-PE administration showed TNF alpha activity using a WEHI-164 cell cytotoxicity assay and IL-6 activity using an IL-6 dependent mouse 7TD1 hybridoma cell proliferation assay. Serum TNF alpha activity peaked at 2 hours after L-MTP-PE administration. Significant differences from lipid-equivalent controls were observed at 2 and 3 hours (P < 0.05). Neutralization of serum TNF alpha activity was accomplished with serial dilutions of rhTNF alpha monoclonal antibody. Serum IL-6 activity peaked at 3 hours after L-MTP-PE administration. Significant differences from lipid-equivalent controls were observed at 2, 3, and 4 hours (P < 0.05). Neutralization of serum IL-6 activity was not achieved with goat anti-rhIL-6 polyclonal antibody. Intravenous L-MTP-PE, but not lipid-equivalent, induces serum TNF alpha and IL-6 activity in normal cats.

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