{"title":"利用能量底物和环核苷酸系统激活剂对人类精子活力的激发试验:II。弱精子症患者精子的研究。","authors":"O Magnus, T Abyholm, I Brekke, K Purvis","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To find differences in effect on sperm motility of agents that increase intracellular cAMP: manganese ion, methyl-isobutyl-xanthine (MIX), 2-deoxyadenosine, glucose, and Mn-MIX and Mn-glucose.</p><p><strong>Design: </strong>Nine men with asthenozoospermia vs. fertile donors.</p><p><strong>Methods: </strong>Sperm was washed in Hepes-buffered saline, motility tested by laser-Doppler technique.</p><p><strong>Results: </strong>Best activation was obtained with Mn and 2-deoxyadenosine; generally poor response to MIX or glucose.</p><p><strong>Conclusions: </strong>Usually, poor endogenous stimulation of adenylyl cyclase, and probably not limited energy supply, is the cause of impaired motility.</p>","PeriodicalId":13990,"journal":{"name":"International Journal of Fertility","volume":"38 2","pages":"123-8"},"PeriodicalIF":0.0000,"publicationDate":"1993-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Provocation testing of human sperm motility using energy substrates and activators of the cyclic nucleotide system: II. Studies on sperm from asthenozoospermic subjects.\",\"authors\":\"O Magnus, T Abyholm, I Brekke, K Purvis\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To find differences in effect on sperm motility of agents that increase intracellular cAMP: manganese ion, methyl-isobutyl-xanthine (MIX), 2-deoxyadenosine, glucose, and Mn-MIX and Mn-glucose.</p><p><strong>Design: </strong>Nine men with asthenozoospermia vs. fertile donors.</p><p><strong>Methods: </strong>Sperm was washed in Hepes-buffered saline, motility tested by laser-Doppler technique.</p><p><strong>Results: </strong>Best activation was obtained with Mn and 2-deoxyadenosine; generally poor response to MIX or glucose.</p><p><strong>Conclusions: </strong>Usually, poor endogenous stimulation of adenylyl cyclase, and probably not limited energy supply, is the cause of impaired motility.</p>\",\"PeriodicalId\":13990,\"journal\":{\"name\":\"International Journal of Fertility\",\"volume\":\"38 2\",\"pages\":\"123-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1993-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Fertility\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Fertility","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Provocation testing of human sperm motility using energy substrates and activators of the cyclic nucleotide system: II. Studies on sperm from asthenozoospermic subjects.
Objective: To find differences in effect on sperm motility of agents that increase intracellular cAMP: manganese ion, methyl-isobutyl-xanthine (MIX), 2-deoxyadenosine, glucose, and Mn-MIX and Mn-glucose.
Design: Nine men with asthenozoospermia vs. fertile donors.
Methods: Sperm was washed in Hepes-buffered saline, motility tested by laser-Doppler technique.
Results: Best activation was obtained with Mn and 2-deoxyadenosine; generally poor response to MIX or glucose.
Conclusions: Usually, poor endogenous stimulation of adenylyl cyclase, and probably not limited energy supply, is the cause of impaired motility.
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