H Nagasawa, F Guo, X C Zhong, B Y Xia, Z S Wang, X J Qui, D Y Wei, E I Chen, J Z Wang, A Suzuki, A Isogai, Y Hori, S Tamura, H Ishizaki
{"title":"家蚕促胸激素的大规模纯化。","authors":"H Nagasawa, F Guo, X C Zhong, B Y Xia, Z S Wang, X J Qui, D Y Wei, E I Chen, J Z Wang, A Suzuki, A Isogai, Y Hori, S Tamura, H Ishizaki","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Prothoracicotropic hormone has been successfully extracted from as many as 2,800,000 silkworm male adult heads and purified partially through an 8-step procedure. Approximately 7 microgram of the crude preparation (\"crude PTTH\") shows activity in the debrained Samia pupa test. A further 4-step chromatographic purification of the \"crude PTTH\" derived from 100,000 Bombyx heads yields 3.4 mg of purified preparation (\"highly purified PTTH\"), of which 7 ng causes adult development in a debrained Samia pupa.</p>","PeriodicalId":21694,"journal":{"name":"Scientia Sinica","volume":"23 8","pages":"1053-60"},"PeriodicalIF":0.0000,"publicationDate":"1980-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Large-scale purification of prothoracicotropic hormone of the silkworm (Bombyx mori).\",\"authors\":\"H Nagasawa, F Guo, X C Zhong, B Y Xia, Z S Wang, X J Qui, D Y Wei, E I Chen, J Z Wang, A Suzuki, A Isogai, Y Hori, S Tamura, H Ishizaki\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Prothoracicotropic hormone has been successfully extracted from as many as 2,800,000 silkworm male adult heads and purified partially through an 8-step procedure. Approximately 7 microgram of the crude preparation (\\\"crude PTTH\\\") shows activity in the debrained Samia pupa test. A further 4-step chromatographic purification of the \\\"crude PTTH\\\" derived from 100,000 Bombyx heads yields 3.4 mg of purified preparation (\\\"highly purified PTTH\\\"), of which 7 ng causes adult development in a debrained Samia pupa.</p>\",\"PeriodicalId\":21694,\"journal\":{\"name\":\"Scientia Sinica\",\"volume\":\"23 8\",\"pages\":\"1053-60\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1980-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientia Sinica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientia Sinica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Large-scale purification of prothoracicotropic hormone of the silkworm (Bombyx mori).
Prothoracicotropic hormone has been successfully extracted from as many as 2,800,000 silkworm male adult heads and purified partially through an 8-step procedure. Approximately 7 microgram of the crude preparation ("crude PTTH") shows activity in the debrained Samia pupa test. A further 4-step chromatographic purification of the "crude PTTH" derived from 100,000 Bombyx heads yields 3.4 mg of purified preparation ("highly purified PTTH"), of which 7 ng causes adult development in a debrained Samia pupa.
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