P Cavatorta, M L Barcellona, M Avitabile, J von Berger, L Masotti
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引用次数: 0
摘要
Tb3+已被测试作为一种探针来确定DNA的G + C组成。DAPI是一种针对a + T碱基的荧光染料，同时被用作互补探针。使用小牛胸腺DNA (G + C = 40%;A + T = 60%)，发现两种染料的结合是独立的，Tb3+每12.5个核苷酸存在一个结合位点，DAPI每8.3个核苷酸存在一个结合位点。因此，同时使用Tb3+和DAPI可以监测A-T, G-C序列。
Terbium as a new probe to detect G+C content of DNA.
Tb3+ has been tested as a probe to determine G + C per cent composition of DNA. DAPI, a fluorescent dye specific for A + T bases, has been employed at the same time as a complementary probe. Using calf thymus DNA (G + C = 40%; A + T = 60%), it has been found that the binding of the two dyes is independent, and that one binding site every 12,5 nucleotides exists for Tb3+ and one binding site every 8.3 nucleotides for DAPI. The simultaneous use of Tb3+ and DAPI allows therefore the monitoring of A-T, G-C sequences.
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