{"title":"乙醇、乙醛、吗啡和纳洛酮对组胺甲基转移酶活性的影响","authors":"George D. Prell, Irene M. Mazurkiewicz-Kwilecki","doi":"10.1016/0364-7722(81)90051-5","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The microassay method of Taylor and Snyder for determining the activity of histamine N-methyltransferase (E.C.2.1.1.8) (HMT), the histamine metabolizing enzyme, was modifies to permit the simultaneous measurement of histamine and HMT activity in the same tissue sample under our experimental conditions.</p></span></li><li><span>2.</span><span><p>2. The synthesis and purification of τ-methyl-[<sup>14</sup>C]-histamine was performed to assess the efficiency of extraction of methylhistamine which in this modified methodology is approximately 80 percent.</p></span></li><li><span>3.</span><span><p>3. The influence of ethanol, acetaldehyde, morphine and naloxone alone and in combination on hypothalamic, thalamus-midbrain and cerebral cortical HMT activity was investigated at concentrations of 0.1 μM to 500 μM.</p></span></li><li><span>4.</span><span><p>4. Ethanol, acetaldehyde and ethanol in the presence of 1 μM morphine failed to markedly influence HMT activity.</p></span></li><li><span>5.</span><span><p>5. At high concentrations morphine strongly inhibited while naloxone enhanced enzyme activity. Morphine's effects on HMT remained unaltered by 1 μM naloxone.</p></span></li><li><span>6.</span><span><p>6. In the present study low concentrations of ethanol and morphine have little effect on HMT activity ‘in vitro’. These observations suggest that changes in HMT activity may play a minor role in the alcohol and morphine-induced central histamine changes observed in our previous ‘in vivo’ studies.</p></span></li></ul></div>","PeriodicalId":20801,"journal":{"name":"Progress in neuro-psychopharmacology","volume":"5 5","pages":"Pages 581-585"},"PeriodicalIF":0.0000,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0364-7722(81)90051-5","citationCount":"10","resultStr":"{\"title\":\"The effects of ethanol, acetaldehyde, morphine and naloxone on histamine methyltransferase activity\",\"authors\":\"George D. Prell, Irene M. Mazurkiewicz-Kwilecki\",\"doi\":\"10.1016/0364-7722(81)90051-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. The microassay method of Taylor and Snyder for determining the activity of histamine N-methyltransferase (E.C.2.1.1.8) (HMT), the histamine metabolizing enzyme, was modifies to permit the simultaneous measurement of histamine and HMT activity in the same tissue sample under our experimental conditions.</p></span></li><li><span>2.</span><span><p>2. The synthesis and purification of τ-methyl-[<sup>14</sup>C]-histamine was performed to assess the efficiency of extraction of methylhistamine which in this modified methodology is approximately 80 percent.</p></span></li><li><span>3.</span><span><p>3. The influence of ethanol, acetaldehyde, morphine and naloxone alone and in combination on hypothalamic, thalamus-midbrain and cerebral cortical HMT activity was investigated at concentrations of 0.1 μM to 500 μM.</p></span></li><li><span>4.</span><span><p>4. Ethanol, acetaldehyde and ethanol in the presence of 1 μM morphine failed to markedly influence HMT activity.</p></span></li><li><span>5.</span><span><p>5. At high concentrations morphine strongly inhibited while naloxone enhanced enzyme activity. Morphine's effects on HMT remained unaltered by 1 μM naloxone.</p></span></li><li><span>6.</span><span><p>6. In the present study low concentrations of ethanol and morphine have little effect on HMT activity ‘in vitro’. These observations suggest that changes in HMT activity may play a minor role in the alcohol and morphine-induced central histamine changes observed in our previous ‘in vivo’ studies.</p></span></li></ul></div>\",\"PeriodicalId\":20801,\"journal\":{\"name\":\"Progress in neuro-psychopharmacology\",\"volume\":\"5 5\",\"pages\":\"Pages 581-585\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0364-7722(81)90051-5\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Progress in neuro-psychopharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0364772281900515\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Progress in neuro-psychopharmacology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0364772281900515","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The effects of ethanol, acetaldehyde, morphine and naloxone on histamine methyltransferase activity
1.
1. The microassay method of Taylor and Snyder for determining the activity of histamine N-methyltransferase (E.C.2.1.1.8) (HMT), the histamine metabolizing enzyme, was modifies to permit the simultaneous measurement of histamine and HMT activity in the same tissue sample under our experimental conditions.
2.
2. The synthesis and purification of τ-methyl-[14C]-histamine was performed to assess the efficiency of extraction of methylhistamine which in this modified methodology is approximately 80 percent.
3.
3. The influence of ethanol, acetaldehyde, morphine and naloxone alone and in combination on hypothalamic, thalamus-midbrain and cerebral cortical HMT activity was investigated at concentrations of 0.1 μM to 500 μM.
4.
4. Ethanol, acetaldehyde and ethanol in the presence of 1 μM morphine failed to markedly influence HMT activity.
5.
5. At high concentrations morphine strongly inhibited while naloxone enhanced enzyme activity. Morphine's effects on HMT remained unaltered by 1 μM naloxone.
6.
6. In the present study low concentrations of ethanol and morphine have little effect on HMT activity ‘in vitro’. These observations suggest that changes in HMT activity may play a minor role in the alcohol and morphine-induced central histamine changes observed in our previous ‘in vivo’ studies.
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