动脉壁糖缀合物的生物合成。8微粒体唾液基转移酶分子类型的分离。

Paroi arterielle Pub Date : 1981-01-01

J Guidollet, P Louisot

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引用次数: 0

摘要

动脉壁中内膜的唾液转移酶活性位于微粒体中。经Triton X-100增溶酶、层析纯化和等电聚焦得到两种同工酶(pHi分别为4.5和6.4)，其中第二种酶占主导地位。主动脉唾液基转移酶对核苷酸敏感，依赖于Mn++，对底物n -乙酰神经氨酸的亲和力为300微米。

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[Glycoconjugate biosynthesis in the arterial wall. VIII. Separation of molecular types in microsomal sialyltransferase].

Sialyltransferase activity of the intima-media of arterial walls is located in microsomes. The solubilization of enzyme by Triton X-100, purification by chromatography and isoelectric focusing lead to two isoenzymes (pHi 4.5 and 6.4), the second is widely predominant. Aortic sialyltransferase is sensitive to nucleotides, Mn++ dependent, and the affinity for substrate N-acetyl-neuraminic acid is 300 microM.

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Paroi arterielle

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