{"title":"确定延胡索酶中催化必需羧基数量的微分标记模型","authors":"Robert C. Seid Jr. , Thomas P. Sakmar","doi":"10.1016/0005-2744(81)90030-9","DOIUrl":null,"url":null,"abstract":"<div><p>We have developed a mathematical model of the nonideal case in which enzymatic activity changes may also result from modification of non-essential groups. As an illustration of this method, the number of essential carboxyl groups in pig heart fumarase (<span>l</span>-malate hydro-lyase, EC 4.2.1.2) was determined by the differential labeling technique. Enzymatic activity was related to the number of modified carboxyl groups according to the model and the results were compatible with the existence of two essential carboxyl groups in fumarase.</p></div>","PeriodicalId":100159,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1981-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2744(81)90030-9","citationCount":"0","resultStr":"{\"title\":\"A differential labeling model for determining the number of catalytically essential carboxyl groups in fumarase\",\"authors\":\"Robert C. Seid Jr. , Thomas P. Sakmar\",\"doi\":\"10.1016/0005-2744(81)90030-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We have developed a mathematical model of the nonideal case in which enzymatic activity changes may also result from modification of non-essential groups. As an illustration of this method, the number of essential carboxyl groups in pig heart fumarase (<span>l</span>-malate hydro-lyase, EC 4.2.1.2) was determined by the differential labeling technique. Enzymatic activity was related to the number of modified carboxyl groups according to the model and the results were compatible with the existence of two essential carboxyl groups in fumarase.</p></div>\",\"PeriodicalId\":100159,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Enzymology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1981-12-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0005-2744(81)90030-9\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Enzymology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0005274481900309\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005274481900309","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A differential labeling model for determining the number of catalytically essential carboxyl groups in fumarase
We have developed a mathematical model of the nonideal case in which enzymatic activity changes may also result from modification of non-essential groups. As an illustration of this method, the number of essential carboxyl groups in pig heart fumarase (l-malate hydro-lyase, EC 4.2.1.2) was determined by the differential labeling technique. Enzymatic activity was related to the number of modified carboxyl groups according to the model and the results were compatible with the existence of two essential carboxyl groups in fumarase.
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