人血浆中黄体酮的直接放射免疫测定。

Endokrinologie Pub Date : 1982-07-01

R Stupnicki, E Kula

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引用次数: 0

摘要

本文描述了一种直接方法，该方法可以在人血浆或其他含有皮质类固醇结合球蛋白(CBG)的物质中进行特异性黄体酮测定，而无需提取。当每管使用20微升血浆时，可达到6 pg/管或0.3 ng/ml的灵敏度。精密度和准确度可与提取方法相媲美。所述方法特别适用于常规测定。

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Direct radioimmunoassay of progesterone in human plasma.

A direct method has been described which enables a specific assay of progesterone without extraction in human plasma or in other material containing corticosteroid binding globulin (CBG). When using 20 microliter of plasma per tube, a sensitivity of 6 pg/tube, or 0.3 ng/ml can be achieved. The precision and accuracy are comparable to those observed in methods involving extraction. The described method is particularly suitable for routine assays.

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Endokrinologie

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