尿激酶放射免疫法定量测定卵巢肿瘤培养物中释放的纤溶酶原激活剂

Birger Åstedt , Lars Holmberg , Ingegerd Lecander , Jan Thorell
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引用次数: 46

摘要

用尿激酶抗血清和31,000道尔顿的放射性碘化dfp灭活尿激酶进行放射免疫测定。对卵巢肿瘤和胎儿肾脏组织培养基的分析显示存在尿激酶样免疫反应性,在凝胶过滤下可以以三种分子形式分离。这三种形式的抑制曲线与纯化的尿激酶相似。测定得到的浓度与酶的活性相对应。在不同年龄的肿瘤培养物中发现了明显的平行曲线,其中三种分子形式的相对浓度不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Radioimmunoassay of urokinase for quantification of plasminogen activators released in ovarian tumour cultures

A radioimmunoassay was developed with an urokinase antiserum and radioiodinated DFP-inactivated urokinase of 31,000 daltons. Assays of tissue culture medium from ovarian tumours and fetal kidneys showed the presence of urokinase-like immunoreactivity, which could be separated in three molecular forms at gel filtration. All three forms showed inhibition curves parallel to that of purified urokinase. The concentration obtained by the assay corresponded to the enzymatic activity. Distinctly parallel curves were found for tumour cultures of various ages in which the three molecular forms differed in their relative concentrations.

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