b型流感嗜血杆菌荚膜多糖的酶联免疫吸附试验(ELISA)检测和测定。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1981-02-01

F W Tiller, E Diener

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引用次数: 0

摘要

建立了一种检测流感嗜血杆菌b型荚膜抗原的直接夹心酶联免疫吸附试验(ELISA)。以聚苯乙烯为固相，过氧化物酶标记兔抗体，检测浓度为0.1 ng/ml的抗原。在1ng ~ 10微克/ml范围内呈线性。体液中流感嗜血杆菌b型荚膜抗原的细微测量可以通过ELISA进行，在这方面优于反免疫电泳和乳胶颗粒凝集。酶联免疫吸附试验(ELISA)有助于研究PRP在实验性Hib感染和人类Hib疾病中的致病作用。

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Haemophilus influenzae type b capsular polysaccharide detection and measurement by an enzyme-linked immunosorbent assay (ELISA).

A direct sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect Haemophilus influenzae type b capsular antigen. Using polystyrene as the solid phase and peroxidase-labelled rabbit antibody the assay detected the antigen in concentrations of 0.1 ng/ml. Linearity was achieved within the range of 1ng to 10 microgram/ml. Subtle measurements of Haemophilus influenzae type b capsular antigen in body fluids are possible through ELISA which is superior to counterimmunoelectrophoresis and latex-particle agglutination in this respect. ELISA should facilitate investigations concerning PRP pathogenic effects in experimental Hib infection as well as in human Hib disease.

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Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie

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