酶联免疫吸附试验(ELISA)诊断人钩端螺旋体病。

Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie Pub Date : 1980-08-01

W J Terpstra, G S Ligthart, G J Schoone

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引用次数: 0

摘要

描述了一种用于钩端螺旋体病血清诊断的酶联免疫吸附测定(ELISA)。使用从单个钩端螺旋体菌株(Wijnberg)的加热培养中制备的抗原，ELISA是一种属特异性试验。显微凝集试验(MAT)作为参考。96例钩端螺旋体病患者95%的血清ELISA和MAT结果一致。在217例对照中发现1例假阳性。该酶联免疫吸附试验具有灵敏度高、特异性强、操作简便等特点。

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Serodiagnosis of human leptospirosis by enzyme-linked-immunosorrbent-assay (ELISA).

An enzyme-linked-immunosorbent-assay (ELISA) is described for the serodiagnosis of leptospirosis. Using an antigen prepared from a heated culture of a single leptospira strain (Wijnberg) the ELISA is a genusspecific test. The microscopic agglutination test (MAT) served as a reference. ELISA and MAT results agreed in 95% of the sera from 96 leptospirosis patients. One false positive was found in 217 controls. The ELISA is sensitive, specific and relatively easy to perform.

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Zentralblatt fur Bakteriologie. 1. Abt. Originale. A: Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie

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