A Kirn, A M Steffan, A Bingen, J Cinqualbre, J L Gendrault
{"title":"[人类Kupffer细胞的分离和培养]。","authors":"A Kirn, A M Steffan, A Bingen, J Cinqualbre, J L Gendrault","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The dissociation of human adult liver parenchyma by collagenase followed by cell separation with centrifugal elutriation allows the Kupffer cells to be isolated. These cells, which may be maintained in culture, retain their main morphological and functional features.</p>","PeriodicalId":10605,"journal":{"name":"Comptes rendus des seances de l'Academie des sciences. Serie D, Sciences naturelles","volume":"291 2","pages":"249-51"},"PeriodicalIF":0.0000,"publicationDate":"1980-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Isolation and culture of human Kupffer cells].\",\"authors\":\"A Kirn, A M Steffan, A Bingen, J Cinqualbre, J L Gendrault\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The dissociation of human adult liver parenchyma by collagenase followed by cell separation with centrifugal elutriation allows the Kupffer cells to be isolated. These cells, which may be maintained in culture, retain their main morphological and functional features.</p>\",\"PeriodicalId\":10605,\"journal\":{\"name\":\"Comptes rendus des seances de l'Academie des sciences. Serie D, Sciences naturelles\",\"volume\":\"291 2\",\"pages\":\"249-51\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1980-09-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comptes rendus des seances de l'Academie des sciences. Serie D, Sciences naturelles\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comptes rendus des seances de l'Academie des sciences. Serie D, Sciences naturelles","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The dissociation of human adult liver parenchyma by collagenase followed by cell separation with centrifugal elutriation allows the Kupffer cells to be isolated. These cells, which may be maintained in culture, retain their main morphological and functional features.