人血浆白蛋白的色谱制备及体外特性研究。

Journal of applied biochemistry Pub Date : 1983-08-01
J H Berglöf, S Eriksson, J M Curling
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引用次数: 0

摘要

白蛋白已通过色谱纯化使用标准化的程序与不同的起始材料取决于以前的分馏。血浆可在低温沉淀和因子IX吸附或IgG分离后直接使用。血浆离心后在Sephadex G-25 Coarse上脱盐,调整pH,沉淀euglo球蛋白,然后在DEAE-和CM-Sepharose CL-6B上离子交换层析。然后进行超滤浓缩,Sephacryl S-200凝胶过滤,最终浓度和配方。获得的白蛋白纯度为99%,含有少于1%的聚集蛋白。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chromatographic preparation and in vitro properties of albumin from human plasma.

Albumin has been purified by chromatography using standardized procedures with different starting materials depending on previous fractionation. Plasma can be used directly after cryoprecipitation and Factor IX adsorption or after isolation of IgG. The plasma was centrifuged and then desalted on Sephadex G-25 Coarse, the pH was adjusted, and the euglobulins were precipitated before ion-exchange chromatography on DEAE- and CM-Sepharose CL-6B. This was followed by concentration by ultrafiltration, gel filtration on Sephacryl S-200, and final concentration and formulation. The albumin obtained was 99% pure and contained less than 1% of aggregated protein.

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