{"title":"腺苷酸抗体的特异性和纯化。","authors":"M A Ali, T M Jacob","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Anti-adenylate antibodies were elicited in rabbits using a conjugate of adenylic acid and bovine serum albumin as immunogen. The specificity of the antibodies was determined by monitoring the inhibition of the binding of [3H]AMP to the antibodies by various nonradioactive nucleic acid components, using a nitrocellulose filter assay. The antibodies were found to be directed against the whole molecule of AMP. They were reactive to polyadenylic acid, RNA, and denatured DNA. The hapten-specific antibodies were isolated by employing an AMP-AH-Sepharose affinity column.</p>","PeriodicalId":14978,"journal":{"name":"Journal of applied biochemistry","volume":"6 3","pages":"126-34"},"PeriodicalIF":0.0000,"publicationDate":"1984-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Specificity and purification of antibodies to adenylic acid.\",\"authors\":\"M A Ali, T M Jacob\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Anti-adenylate antibodies were elicited in rabbits using a conjugate of adenylic acid and bovine serum albumin as immunogen. The specificity of the antibodies was determined by monitoring the inhibition of the binding of [3H]AMP to the antibodies by various nonradioactive nucleic acid components, using a nitrocellulose filter assay. The antibodies were found to be directed against the whole molecule of AMP. They were reactive to polyadenylic acid, RNA, and denatured DNA. The hapten-specific antibodies were isolated by employing an AMP-AH-Sepharose affinity column.</p>\",\"PeriodicalId\":14978,\"journal\":{\"name\":\"Journal of applied biochemistry\",\"volume\":\"6 3\",\"pages\":\"126-34\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of applied biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of applied biochemistry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Specificity and purification of antibodies to adenylic acid.
Anti-adenylate antibodies were elicited in rabbits using a conjugate of adenylic acid and bovine serum albumin as immunogen. The specificity of the antibodies was determined by monitoring the inhibition of the binding of [3H]AMP to the antibodies by various nonradioactive nucleic acid components, using a nitrocellulose filter assay. The antibodies were found to be directed against the whole molecule of AMP. They were reactive to polyadenylic acid, RNA, and denatured DNA. The hapten-specific antibodies were isolated by employing an AMP-AH-Sepharose affinity column.
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