不同方法测定大鼠肝脏和细叶草苯丙氨酸羟化酶活性的比较。

R M Fink, E F Elstner
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引用次数: 1

摘要

比较了三种不同的测定苯丙氨酸羟化酶活性的方法:a)在苯丙氨酸存在下酪氨酸浓度增加的差异光度法;b)对形成的[14C]酪氨酸进行薄层色谱分离和闪烁计数;c)衍生氨基酸的高效液相色谱分离和荧光光谱定量。通过对大鼠肝脏和细叶草中苯丙氨酸羟化酶活性的比较,可以清楚地看出,b)和c)中只有大鼠肝脏含有该酶活性,尽管在分光光度测试中发现细叶草制剂具有伪活性(A)。高效液相色谱法是直接测定酪氨酸从而测定苯丙氨酸羟化酶活性的最快、最可靠和最方便的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of different methods for the determination of phenylalanine hydroxylase activity in rat liver and Euglena gracilis.

Three different methods for the determination of phenylalanine hydroxylase activity have been compared: a) Differential photometric assay of the increase in tyrosine concentration in the presence of phenylalanine; b) Product separation by thin layer chromatography and scintillation counting of the [14C]tyrosine formed; c) HPLC separation and spectrofluorometric quantification of derivatized amino acids. A comparison of the activities of phenylalanine hydroxylase in rat liver and Euglena gracilis clearly showed that only rat liver contains this enzymic activity as shown by methods b) and c) although pseudo-activity of Euglena gracilis preparations was found during the spectrophotometric test a). The HPLC method proved to be the fastest, most reliable and convenient method for direct tyrosine determination and thus for measuring phenylalanine hydroxylase activity.

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