[百日咳毒素刺激脑腺苷酸环化酶，诱导40,000道尔顿膜蛋白的ADP核糖基化]。

Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-01-01

G Berthillier, F Megret, J E Alouf, A Monneron

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引用次数: 0

摘要

大鼠脑突触体膜腺苷酸环化酶活性被福斯克林强烈激活。本研究证明:1。当将GTP或其类似物添加到forskolin中时，可以将酶的刺激降低40 - 60%;2. 该百日咳毒素(TP)，在NAD的存在下，adp核糖基化一个40000道尔顿表观分子量的单一膜蛋白，以严格伴随的方式。该蛋白的沉降系数为3.6,Stokes半径为6 nm。抗TP的免疫血清可阻断毒素的nadh -糖水解酶活性。

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[Pertussis toxin stimulates brain adenyl cyclase and induces ADP ribosylation of a 40,000 dalton membrane protein].

The adenylate cyclase activity of Rat brain synaptosomal membranes is strongly activated by forskolin. This study demonstrates: 1. That GTP or its analogs, when added to forskolin, decrease the enzyme stimulation by a factor of 40 to 60%; 2. That Pertussis toxin (TP), in the presence of NAD, ADP-ribosylates a single membrane protein of 40,000 dalton apparent molecular weight in a strictly concomitant manner. This Lubrol-soluble protein has a sedimentation coefficient of 3.6 and a Stokes radius of 6 nm. An immune serum against TP blocks the NAD-glycohydrolase activity of the toxin.

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Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie

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