[UDP半乳糖的存在:培养神经元外表面的半乳糖转移酶活性]。

Y Matsui, D Lombard, B Hoflack, S Harth, R Massarelli, P Mandel, H Dreyfus
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引用次数: 0

摘要

证实了鸡胚半脑神经元外表面存在udp -半乳糖半乳糖转移酶活性。[14C]-半乳糖被转移到神经元表面的细胞底物和与Sepharose beads结合的外源底物(ovomucoïd)上。外半乳糖转移酶的活性不是由于细胞内酶渗漏到培养培养基中,也不是由于利用UDP-[14C]-半乳糖水解获得的[14C]-半乳糖的细胞内酶活性。分析了这些外半乳糖转移酶的动力学。这些结果支持了神经细胞表面之间通过糖缀合物或其生物合成酶进行交流和/或相互作用的假设。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Existence of UDP galactose: galactosyltransferase activity at the external surface of cultured neurons].

The existence of UDP-galactose:galactosyltransferase activities at the external surface of cultured neurons from chick embryo hemispheres, was demonstrated. [14C]-galactose was transferred upon cellular substrates present on the neuronal surface and upon exogenous substrates (ovomucoïd) bound to Sepharose beads. The activity of ectogalactosyltransferase was not due to a leakage of intracellular enzymes into the incubation medium nor to an intracellular enzymatic activity utilizing [14C]-galactose obtained from the hydrolysis of UDP-[14C]-galactose. Kinetics of these ectogalactosyltransferases were analysed. The results support the hypothesis of a communication and/or interaction among the nerve cell surfaces via glycoconjugates or their biosynthetic enzymes.

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