[钠钾atp酶内源性抑制剂的纯化]。

Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie Pub Date : 1983-02-07

J F Cloix, E D Miller, M G Pernollet, M A Devynck, P Meyer

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引用次数: 0

摘要

从健康人血浆中，采用凝胶过滤-阴离子交换层析-反相高效液相色谱法制备Na+， K+- atp酶抑制剂。这种低分子量(小于1500道尔顿)的抑制剂是一种具有阴离子电荷的物质，在反相上被吸收。它抑制Na+， K+- atp酶活性和3h -华巴因在人红细胞上的结合。

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[Purification of an endogenous inhibitor of sodium-potassium-ATPase].

From human plasma of healthy subject, an inhibitor of Na+, K+-ATPase was prepared, using a gel filtration followed by anion exchange chromatography and by HPLC on reverse phase. This low molecular weight (less than 1,500 dalton) inhibitor is a substance which possesses anionic charges, and is absorbed on reverse phase. It inhibits Na+, K+-ATPase activity and the 3H-ouabain binding on human red blood cells.

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Comptes rendus des seances de l'Academie des sciences. Serie III, Sciences de la vie

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