转录截断DNA片段的大肠杆菌RNA聚合酶的末端链转换

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis Pub Date : 1981-10-27 DOI:10.1016/0005-2787(81)90056-3

Ben A. Oostra, Annika C. Arnberg, Geert Ab, Max Gruber

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引用次数: 6

摘要

当转录含有大肠杆菌启动子和rrnE操纵子第一部分的限制性内切片段时，RNA聚合酶全酶只在启动子上开始。除了径流转录本外，末端链开关还可形成比模板大的分子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Terminal strand-switching of E. coli RNA polymerase transcribing a truncated DNA fragment

When transcribing a restriction fragment containing the promoters and the first part of the rrnE operon of Escherichia coli, RNA polymerase holoenzyme starts exclusively on the promoters. Besides run-off transcripts, molecules longer than template-size are formed by terminal strand switch.

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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis

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