Heterotransplantation of natural bovine lymphosarcoma to nude mice

Nude mice of BALB/c background were utilized for the heterotransplantation of tissues derived from 4 forms of natural bovine lymphosarcoma. The growing tumors have been established from adult case tumor (6 out of 22 attempts), from calf case tumor (2 out of 5 attempts), from skin case tumor (18 out of 30 attempts) and from thymic case tumor (3 out of 11 attempts). In all, successful tumor growth was observed in 29 out of 68 transplanted natural bovine lymphosarcoma cells. Transplanted tumors from skin case grew fast, metastasized and then became lethal to the host. However, transplanted adult case tumors regressed and no spread of tumor cells was observed.