[Na+激活、速尿抑制大鼠肠黏膜atp酶活性的检测]。

Acta biologica et medica Germanica Pub Date : 1982-01-01
D Luppa, F Müller
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引用次数: 0

摘要

在大鼠小肠粘膜匀浆中发现了一种不敏感的、依赖于Mg++的、受Na+刺激的、被速尿抑制的atp酶活性。该atp酶活性的亚细胞定位研究了分离纯化的刷状边界和基底外侧质膜。结果表明,上皮细胞内Na+活化和(Na+K+)活化的atp酶分布几乎相同。在四氧嘧啶和链脲佐菌素糖尿病条件下,两种atp酶活性的增加只在基底外侧质膜中发现。这些观察结果与肠道吸收的对流模型非常吻合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Detection of a Na+-activated, furosemide-inhibited ATPase activity in rat intestinal mucosa].

An ouabain-insensitive, Mg++-dependent, Na+-stimulated ATPase activity which is inhibited by furosemide was found in mucosal homogenate of rat small intestine. The subcellular localization of this ATPase activity was studied by means of isolated purified brush borders and basolateral plasma membranes. The results suggest a nearly identical distribution of Na+-activated and (Na+K+)-activated ATPase within the epithelial cells. Under conditions of alloxan and streptozotocin diabetes an increase of both ATPase activities can be found only in the basolateral plasma membranes. These observations agree well with the convective model of intestinal absorption.

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