[在各种形式的热扰动下虹鳟鱼和鲱鱼血浆中12种酶的活性]。

Revue canadienne de biologie Pub Date : 1980-09-01
H Perrier, G Llaurens, C Perrier, G Pérès, J Gras
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引用次数: 0

摘要

测定了不同水温条件下虹鳟鱼和丹青鱼血浆中鸟氨酸氨甲酰转移酶、酸性磷酸酶、碱性磷酸酶、亮氨酸氨基肽酶、肌酸激酶、淀粉酶、谷草乙酸转氨酶、谷丙转氨酶、乳酸脱氢酶、羟丁酸脱氢酶、谷氨酸脱氢酶和苹果酸脱氢酶的水平。在虹鳟鱼中,从12度到21度的热休克会增加一些酶的活性,而温度升高到死前阶段会导致酶水平发生非常重要的变化。在美国,从12度到28度的热冲击比从12度到25度的热冲击更能引起酶活性的变化。在驯化于25度的田鼠中,与12度对照动物相比,各种酶水平均有所提高。水中钾含量高会引起酶水平的复杂变化。对热干扰最敏感的酶是无论侵略形式如何都增加的GOT和GPt转氨酶,热干扰适度时增加的淀粉酶,强热胁迫时增加的碱性磷酸酶和苹果酸脱氢酶。对这些酶的研究被推荐用于观察生活在人工加热水域的鱼类的状态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Activities of twelve enzymes in the blood plasma of rainbow trout and tench subjected to various forms of thermal disturbance].

The levels of ornithine carbamyl transferase, acid phosphatase, alkaline phosphatase, leucine amino peptidase, creatine kinase, amylase, glutamic oxalacetic transaminase, glutamic pyruvic transaminase, lactate deshydrogenase, hydroxybutyrate deshydrogenase, glutamate deshydrogenase and malate deshydrogenase were determined in the plasma of Rainbow Trout and Tench submitted to water temperature increases. In the Rainbow Trout a thermal shock from 12 to 21 degrees, increases activities of some enzymes while temperature increase up to pre-mortem stage causes very important changes in enzymatic levels. In the Tench a thermal shock from 12 to 28 degrees causes more changes of enzymatic activities than a shock from 12 to 25 degrees. In Tench acclimated to 25 degrees, various enzyme levels are increased in comparison with 12 degrees control animals. A high potassium level in water causes complex changes in enzyme levels. The most sensitive enzymes to thermal disturbance are GOT and GPt transaminases which increase whatever the aggression form, amylase when thermal disturbance is moderate, alkaline phosphatase and malate deshydrogenase in case of strong thermal stress. The study of these enzymes is recommended for watching the state of fishes living in artificially heated waters.

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