无血清培养基中的大鼠肝上皮细胞培养:原代培养和表达分化功能的衍生细胞系。

In Vitro Pub Date : 1984-10-01 DOI:10.1007/BF02618294

M Chessebeuf, P Padieu

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引用次数: 14

摘要

大鼠肝上皮细胞外植于由Ham’s F10基础培养基和牛白蛋白吸附的游离脂肪酸组成的无血清培养基(SFM)中，成功地培养出原代培养，然后培养出表达肝功能的长期细胞系;糖皮质激素对l -酪氨酸转氨酶的诱导作用，黄体酮代谢的肝脏模式，以及小鼠初级胆油酸的生物合成;高等脊椎动物常见的鹅去氧胆酸和胆酸以及大鼠胆汁中特有的-胆酸。

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Rat liver epithelial cell cultures in a serum-free medium: primary cultures and derived cell lines expressing differentiated functions.

Rat liver epithelial cells explanted in a serum-free medium (SFM) composed of Ham's F10 basal medium plus free fatty acids adsorbed on bovine albumin gave successful rise to primary cultures and then to long-term cell lines that expressed liver functions; induction of L-tyrosine aminotransferase by glucocorticoids, hepatic pattern of progesterone metabolism, and biosynthesis of murine primary bile acids; chenodeoxycholic and cholic acid common to higher vertebrates and alpha-muricholic acid specific of the rat bile.

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In Vitro

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