N L Banik, E L Hogan, L J Whetstine, J D Balentine
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引用次数: 19
摘要
将1.0 ml pH为7.4的10% CaCl2溶液滴在暴露的脊髓上，产生钙诱导的脊髓病。应用钙后检测脊髓蛋白的变化。SDS-PAGE蛋白分析显示，在8小时至5天的时间内，神经丝、微管和胶质丝蛋白逐渐丢失。髓磷脂蛋白的大量损失也很明显。观察到的蛋白质变化与超微结构变化密切相关，与先前发现的物理创伤相似。这些观察结果表明，Ca2+在Ca2+诱导的脊髓病和脊髓损伤的轴突和髓鞘变性中起关键作用，可能是通过激活蛋白酶(s)。
Changes in myelin and axonal proteins in CaCl2-induced myelopathy in rat spinal cord.
Calcium-induced myelopathy was produced in rats by dripping 1.0 ml of a 10% solution of CaCl2 at pH 7.4 upon exposed spinal cord. Changes in spinal cord proteins were examined following application of calcium. Analysis of proteins by SDS-PAGE revealed progressive losses of neurofilament, microtubular, and glial filament proteins over a period of 8 hours to 5 days. Large losses of myelin proteins were also evident. The protein alterations observed correlate well with ultrastructural changes and resemble those previously found with physical trauma. These observations indicate that Ca2+ plays a pivotal role, possibly by activating proteinase(s), in the degeneration of axons and myelin sheath in both Ca2+-induced myelopathy and spinal cord injury.
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