{"title":"肝胆疾病患者血清中3β-羟基-5-胆烯酸的测定及其糖醛酸和硫酸盐缀合物","authors":"Hajime Takikawa , Hideaki Otsuka , Tomoe Beppu , Yousuke Seyama","doi":"10.1016/0006-2944(85)90016-X","DOIUrl":null,"url":null,"abstract":"<div><p>3β-Hydroxy-5-cholenoic acid in the serum of control subjects and 62 patients with various hepatobiliary diseases was quantitated by mass fragmentography after separation into nonglucuronidated-nonsulfated, glucuronidated, and sulfated fractions. Deuterium-labeled deoxycholic acid and its glucuronide and sulfate were used as internal standards. Mean concentrations of total 3β-hydroxy-5-cholenoic acid in serum (μmole/liter) were as follows: Control subjects (14), 0.184; obstructive jaundice (15), 6.783; liver cirrhosis, compensated (12), 0.433, and decompensated (12), 1.636; chronic hepatitis (12), 0.241; and acute hepatitis (11), 2.364. Most of the 3β-hydroxy-5-cholenoic acid was glucuronidated or sulfated. Only in patients with obstructive jaundice did glucuronidation (60 ± 14%) exceed sulfation (31 ± 14%), sulfation exceeding glucuronidation in the others. The UDP-glucuronyltransferase might have different substrate specificities for 3β-hydroxy-5-cholenoic acid and other common bile acids, especially in the cholestatic state.</p></div>","PeriodicalId":8781,"journal":{"name":"Biochemical medicine","volume":"33 3","pages":"Pages 393-400"},"PeriodicalIF":0.0000,"publicationDate":"1985-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0006-2944(85)90016-X","citationCount":"10","resultStr":"{\"title\":\"Determination of 3β-hydroxy-5-cholenoic acid in serum of hepatobiliary diseases—Its glucuronidated and sulfated conjugates\",\"authors\":\"Hajime Takikawa , Hideaki Otsuka , Tomoe Beppu , Yousuke Seyama\",\"doi\":\"10.1016/0006-2944(85)90016-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>3β-Hydroxy-5-cholenoic acid in the serum of control subjects and 62 patients with various hepatobiliary diseases was quantitated by mass fragmentography after separation into nonglucuronidated-nonsulfated, glucuronidated, and sulfated fractions. Deuterium-labeled deoxycholic acid and its glucuronide and sulfate were used as internal standards. Mean concentrations of total 3β-hydroxy-5-cholenoic acid in serum (μmole/liter) were as follows: Control subjects (14), 0.184; obstructive jaundice (15), 6.783; liver cirrhosis, compensated (12), 0.433, and decompensated (12), 1.636; chronic hepatitis (12), 0.241; and acute hepatitis (11), 2.364. Most of the 3β-hydroxy-5-cholenoic acid was glucuronidated or sulfated. Only in patients with obstructive jaundice did glucuronidation (60 ± 14%) exceed sulfation (31 ± 14%), sulfation exceeding glucuronidation in the others. The UDP-glucuronyltransferase might have different substrate specificities for 3β-hydroxy-5-cholenoic acid and other common bile acids, especially in the cholestatic state.</p></div>\",\"PeriodicalId\":8781,\"journal\":{\"name\":\"Biochemical medicine\",\"volume\":\"33 3\",\"pages\":\"Pages 393-400\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1985-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0006-2944(85)90016-X\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemical medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/000629448590016X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/000629448590016X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Determination of 3β-hydroxy-5-cholenoic acid in serum of hepatobiliary diseases—Its glucuronidated and sulfated conjugates
3β-Hydroxy-5-cholenoic acid in the serum of control subjects and 62 patients with various hepatobiliary diseases was quantitated by mass fragmentography after separation into nonglucuronidated-nonsulfated, glucuronidated, and sulfated fractions. Deuterium-labeled deoxycholic acid and its glucuronide and sulfate were used as internal standards. Mean concentrations of total 3β-hydroxy-5-cholenoic acid in serum (μmole/liter) were as follows: Control subjects (14), 0.184; obstructive jaundice (15), 6.783; liver cirrhosis, compensated (12), 0.433, and decompensated (12), 1.636; chronic hepatitis (12), 0.241; and acute hepatitis (11), 2.364. Most of the 3β-hydroxy-5-cholenoic acid was glucuronidated or sulfated. Only in patients with obstructive jaundice did glucuronidation (60 ± 14%) exceed sulfation (31 ± 14%), sulfation exceeding glucuronidation in the others. The UDP-glucuronyltransferase might have different substrate specificities for 3β-hydroxy-5-cholenoic acid and other common bile acids, especially in the cholestatic state.
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