大鼠组织中caligulin样免疫反应性的免疫细胞化学定位。

Medical biology Pub Date : 1986-01-01
O Nilsson, A Dahlström, S Bööj, L Rosengren, K Haglid, J Baudier, D Gérard
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引用次数: 0

摘要

利用兔培养的抗牛脑caligulin抗体,研究了caligulin-li在大鼠中枢和外周组织中的细胞定位。采用免疫荧光和免疫过氧化物酶技术对多聚甲醛固定组织中的caligulin-LI进行了鉴定。大脑皮层、基底神经节和脑干的某些神经元含有caligulin-LI。在小脑中,大多数浦肯野细胞被标记有定位于树突、核周和轴突的免疫反应物质。胃肠道中奥尔巴赫神经丛和迈斯纳神经丛的部分神经元含有caligulin-LI。坐骨神经的结扎引起了近端和远端的免疫反应物质的积累。caligulin-LI的非神经元定位也可以被证实,例如,在肾小管系统的部分,在朗格汉斯岛和某些肠内分泌细胞中。caligulin- li特异定位于大鼠大脑和胃肠道的部分神经元,但不是全部神经元,这表明caligulin在中枢和周围神经机制中具有特异性功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunocytochemical localisation of caligulin-like immunoreactivity in rat tissues.

The cellular localisation of caligulin-like immunoreactivity (caligulin-LI) in rat central and peripheral tissues was studied using antibodies against bovine brain caligulin raised in rabbits. Both immunofluorescence and immunoperoxidase techniques were used to demonstrate caligulin-LI in paraformaldehyde fixed tissues. Certain neurons in the cerebral cortex, basal ganglia and brain stem contained caligulin-LI. In the cerebellum a majority of Purkinje cells were labelled with immunoreactive material localised to dendrites, perikarya and axons. In the gastrointestinal tract some neurons in Auerbach's and Meissner's plexa contained caligulin-LI. Ligation of the sciatic nerve caused accumulation of immunoreactive material both proximal and distal to the crush. A non-neuronal localisation of caligulin-LI could also be demonstrated, e.g., in parts of the renal tubular system, in the islets of Langerhans and in certain enteroendocrine cells. The specific localisation of caligulin-LI in some but not all neurons of the rat brain and gastrointestinal tract suggests a specific function of caligulin in central and peripheral nervous mechanisms.

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