{"title":"颗粒棘球蚴原棘突在+4℃和+37℃几种培养基中的体外存活。","authors":"N Casado, F Rodriguez-Caabeiro, S Hernandez","doi":"10.1007/BF00931155","DOIUrl":null,"url":null,"abstract":"<p><p>As a first step towards hydatid disease control, the in vitro survival of protoscolices of Echinococcus granulosus was investigated for various media and temperatures. Higher percentage survival was obtained than previously reported: at 4 degrees C, 100% survival was obtained for 20 days in medium 199 (GIBCO) and for 25 days in hydatid fluid from the host of origin. Maximal survival was 30% at 55 days in these conditions. Flame cell activity was the criterion of choice for viability. At 37 degrees C survival rates were lower and morphological changes in protoscolices were observed.</p>","PeriodicalId":76856,"journal":{"name":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","volume":"72 2","pages":"273-8"},"PeriodicalIF":0.0000,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00931155","citationCount":"24","resultStr":"{\"title\":\"In vitro survival of Echinococcus granulosus protoscolices in several media, at +4 degrees C and +37 degrees C.\",\"authors\":\"N Casado, F Rodriguez-Caabeiro, S Hernandez\",\"doi\":\"10.1007/BF00931155\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>As a first step towards hydatid disease control, the in vitro survival of protoscolices of Echinococcus granulosus was investigated for various media and temperatures. Higher percentage survival was obtained than previously reported: at 4 degrees C, 100% survival was obtained for 20 days in medium 199 (GIBCO) and for 25 days in hydatid fluid from the host of origin. Maximal survival was 30% at 55 days in these conditions. Flame cell activity was the criterion of choice for viability. At 37 degrees C survival rates were lower and morphological changes in protoscolices were observed.</p>\",\"PeriodicalId\":76856,\"journal\":{\"name\":\"Zeitschrift fur Parasitenkunde (Berlin, Germany)\",\"volume\":\"72 2\",\"pages\":\"273-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1986-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1007/BF00931155\",\"citationCount\":\"24\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift fur Parasitenkunde (Berlin, Germany)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1007/BF00931155\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur Parasitenkunde (Berlin, Germany)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/BF00931155","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In vitro survival of Echinococcus granulosus protoscolices in several media, at +4 degrees C and +37 degrees C.
As a first step towards hydatid disease control, the in vitro survival of protoscolices of Echinococcus granulosus was investigated for various media and temperatures. Higher percentage survival was obtained than previously reported: at 4 degrees C, 100% survival was obtained for 20 days in medium 199 (GIBCO) and for 25 days in hydatid fluid from the host of origin. Maximal survival was 30% at 55 days in these conditions. Flame cell activity was the criterion of choice for viability. At 37 degrees C survival rates were lower and morphological changes in protoscolices were observed.
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