人血浆中的天然细胞毒素:与细胞毒性脂蛋白相关的磷脂的分离和表征。

Molecular toxicology Pub Date : 1987-04-01
J A Prezioso, P H Koo
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引用次数: 0

摘要

正常人血浆中大多数热稳定的天然细胞毒素经凝胶过滤分离成两个活性部分:α 2-巨球蛋白(α 2M)和α 2M前池。α - 2M前池似乎也包含了人血浆中的大部分磷脂。通过超离心进一步纯化α - 2M前池,发现含有细胞毒性脂蛋白,代表池中大部分的细胞毒素活性。与α - 2M前池相关的磷脂和纯化脂蛋白在缓冲盐水中抵抗透析,但可以用有机溶剂提取。两种主要的磷脂(化合物A和B)均表现出细胞毒活性。化合物A在体外对肿瘤细胞具有剂量依赖性、非物种特异性的生长抑制或细胞毒性作用。它是一种类似卵磷脂的化合物,但除多不饱和卵磷脂外,比任何卵磷脂标准品都更有效。讨论了某些卵磷脂样化合物对脂蛋白的细胞毒活性及其在抗癌中的作用的可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Natural cytotoxins in human plasma: isolation and characterization of phospholipids associated with cytotoxic lipoproteins.

Most of the heat-stable natural cytotoxins in normal human plasma were fractionated by gel filtration into two active fractions: the alpha 2-macroglobulin (alpha 2M) and pre-alpha 2M pools. The pre-alpha 2M pool also appeared to contain most of the phospholipids in human plasma. The pre-alpha 2M pool was further purified by ultracentrifugation and was shown to contain cytotoxic lipoproteins that represented most of the cytotoxin activity in the pool. The phospholipids associated with the pre-alpha 2M pool and purified lipoproteins resisted dialysis in a buffered saline but were extractable by organic solvents. Two major phospholipids (compounds A and B) were extracted, and both exhibited cytotoxic activities. Compound A exerted dose-dependent, species-nonspecific growth-inhibitory or cytotoxic effects on tumor cells in vitro. It was a lecithin-like compound but was more potent than any of the lecithin standards tested, except for a polyunsaturated lecithin. The possibilities that certain lecithin-like compound(s) contribute significantly to the cytotoxic activity of the lipoproteins and their role in cancer resistance are discussed.

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