Elizabeth R. Hall, Caryl L. Lane, D. Scott Linthicum
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引用次数: 4
摘要
已制备并鉴定了血栓素B2 (TXB2)单克隆抗体。使用这些单克隆抗体中的一种，已经开发了液体和固相放射免疫测定。两种方法在测定11份血清样本的TXB2时结果相似;然而，在B/B0 = 90%时，固相法比液相法更敏感(即63 pg/ml vs 19 pg/ml)。尽管两种检测系统的敏感性不同，但PGD2、PGE2、PGF2α和6酮- pgf1 α单克隆抗体的交叉反应性是相同的。
Monoclonal antibody against TXB2: Its use in solid and liquid phase radirmmunoassays
Monoclonal antibodies to thromboxane B2 (TXB2) have been produced and characterized. Both liquid and solid phase radio immunoassays have been developed using one of these monoclonal antibodies. The two assays gave similar results when used to quantitate TXB2 in 11 serum samples; however the solid phase assay was more sensitive than the liquid phase assay (i.e., 63 pg/ml vs 19 pg/ml) at a B/B0 = 90%). Despite a difference in the sensitivity of the two assay systems, the cross-reactivity of the monoclonal antibody for PGD2, PGE2, PGF2α and 6 keto-PGF1α was the same.
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