{"title":"不透明蛋白2对AGPL2的转激活是甜玉米淀粉积累和籽粒硬度的主要原因。","authors":"Jianjian Chen, Bin Wang, Xiaolong Chen, Xianwen Zhang, Huabing Lu, Xuegao Lv, Guihua Lv, Ruiqiu Fang","doi":"10.1093/jxb/eraf426","DOIUrl":null,"url":null,"abstract":"<p><p>Kernel hardness is an important indicator of sweet corn quality. The mechanism underlying kernel hardness formation and regulation remains poorly understood. In this study, we showed that the endosperm composed of starch and protein was positively correlated with kernel hardness in sweet corn. AGPL2, encoding a subunit of AGPase, was differentially expressed in endosperm of different sweet corn cultivars and was responsible for residual AGPase activity and starch biosynthesis in sweet corn. Opaque2 (O2), a key regulatory transcriptional factor for endosperm development, directly bound to the promoter of AGPL2 and activates its expression, resulting in the accumulation of starch in sweet corn. Taken together, these results unraveled a novel mechanism whereby O2-AGPL2 module participate in regulation of kernel hardness in sweet corn.</p>","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transactivation of AGPL2 by Opaque2 is responsible for starch accumulation and kernel hardness in sweet corn.\",\"authors\":\"Jianjian Chen, Bin Wang, Xiaolong Chen, Xianwen Zhang, Huabing Lu, Xuegao Lv, Guihua Lv, Ruiqiu Fang\",\"doi\":\"10.1093/jxb/eraf426\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Kernel hardness is an important indicator of sweet corn quality. The mechanism underlying kernel hardness formation and regulation remains poorly understood. In this study, we showed that the endosperm composed of starch and protein was positively correlated with kernel hardness in sweet corn. AGPL2, encoding a subunit of AGPase, was differentially expressed in endosperm of different sweet corn cultivars and was responsible for residual AGPase activity and starch biosynthesis in sweet corn. Opaque2 (O2), a key regulatory transcriptional factor for endosperm development, directly bound to the promoter of AGPL2 and activates its expression, resulting in the accumulation of starch in sweet corn. Taken together, these results unraveled a novel mechanism whereby O2-AGPL2 module participate in regulation of kernel hardness in sweet corn.</p>\",\"PeriodicalId\":15820,\"journal\":{\"name\":\"Journal of Experimental Botany\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2025-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Experimental Botany\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/jxb/eraf426\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Experimental Botany","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/jxb/eraf426","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Transactivation of AGPL2 by Opaque2 is responsible for starch accumulation and kernel hardness in sweet corn.
Kernel hardness is an important indicator of sweet corn quality. The mechanism underlying kernel hardness formation and regulation remains poorly understood. In this study, we showed that the endosperm composed of starch and protein was positively correlated with kernel hardness in sweet corn. AGPL2, encoding a subunit of AGPase, was differentially expressed in endosperm of different sweet corn cultivars and was responsible for residual AGPase activity and starch biosynthesis in sweet corn. Opaque2 (O2), a key regulatory transcriptional factor for endosperm development, directly bound to the promoter of AGPL2 and activates its expression, resulting in the accumulation of starch in sweet corn. Taken together, these results unraveled a novel mechanism whereby O2-AGPL2 module participate in regulation of kernel hardness in sweet corn.
期刊介绍:
The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology.
Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.