高压氧与臭氧联合治疗对缺血再灌注损伤生化指标影响的实验研究

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-09-04 DOI:10.1016/j.bbrep.2025.102232

Asli Datli , Ozgur Pilanci , Savas Ilgezdi , Saadet Pilten Guzel , Mehmet Bozkurt

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引用次数: 0

摘要

目的通过氧化应激标志物的分析，比较高压氧治疗（HBO）和臭氧治疗（OT）对大鼠腹直肌（RAM）皮瓣缺血再灌注损伤的影响。方法36只成年Sprague-Dawley大鼠分为HBO组、OT组、HBO + OT组、对照组和假手术组。将RAM皮瓣诱导缺血再灌注损伤4 h，采用ELISA法测定第1天和第7天血清样品的髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）和丙二醛（MDA）水平。结果HBO + OT组第7天sod水平明显升高（p = 0.044）。假手术组和对照组的MPO水平在第7天显著升高（p = 0.049, p = 0.025）。对照组、OT组和HBO组MDA水平在第7天显著升高（p = 0.028）。虽然HBO和OT降低了氧化应激，但随着时间的推移，HBO + OT联合治疗似乎提高了氧化应激标志物。结论高压氧和OT治疗在减少缺血再灌注损伤时氧化应激有各自的疗效。虽然两种疗法都能减少氧化损伤，但将它们结合起来可能会提供额外的保护。

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Combined effects of hyperbaric oxygen and ozone therapy on biochemical markers in ischemia-reperfusion injury: An experimental study

Objective

This study aimed to compare the effects of hyperbaric oxygen therapy (HBO) and ozone therapy (OT) on ischemia-reperfusion injury in Sprague-Dawley rat rectus abdominis muscle (RAM) flaps through the analysis of oxidative stress markers.

Methods

This experimental study involved 36 adult Sprague-Dawley rats divided into five groups: HBO, OT, HBO + OT, Control, and Sham. Ischemia-reperfusion injury was induced in RAM flaps for 4 h. Levels of myeloperoxidase (MPO), superoxide dismutase (SOD), and malondialdehyde (MDA) were measured in serum samples on the 1st and 7th days using the ELISA method.

Results

SOD levels increased significantly on the 7th day in the HBO + OT group (p = 0.044). MPO levels were significantly elevated in the Sham and Control groups by day 7 (p = 0.049, p = 0.025). MDA levels increased significantly in the Control, OT, and HBO groups on day 7 (p = 0.028). While HBO and OT reduced oxidative stress, the combined HBO + OT therapy appeared to elevate oxidative stress markers over time.

Conclusion

HBO and OT therapies show individual efficacy in reducing oxidative stress during ischemia-reperfusion injury. While both therapies reduced oxidative damage, combining them might offer additional protection.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.