评价线粒体替代肽MTALTND4对基因表达的影响

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-08-27 DOI:10.1016/j.bbrep.2025.102223

Ludovic Nadeau-Lachance, Thierry Choquette, Hajar Hosseini Khorami, Annie Angers, Sophie Breton

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引用次数: 0

摘要

•这是一项利用微阵列、RNA-seq和RT-qPCR技术探索线粒体替代肽MTALTND4对两种不同培养基中基因表达影响的初步研究。•MiR05培养基中的微阵列显示，外源MTALTND4处理可能改变基因表达，应答基因大多与细胞代谢有关。•相反，DMEM低糖中的RNA-seq表明MTALTND4对基因表达的影响可以忽略不计。•MiR05或DMEM低糖的RT-qPCR表明培养基影响基因调控。•本研究强调了在检查对细胞的生理影响时采用多种方法的重要性，以及仔细选择合适的培养基以准确解释细胞反应和表达数据的必要性。

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Evaluating the effect of the mitochondrial alternative peptide MTALTND4 on gene expression

• This is a preliminary study exploring the effect of the mitochondrial alternative peptide MTALTND4 on gene expression in two different culture media using microarrays, RNA-seq and RT-qPCR.

• Microarrays in MiR05 medium suggest that exogenous treatment with MTALTND4 may alter gene expression and that responsive genes are mostly related to cell metabolism.

• Conversely, RNA-seq in DMEM low glucose suggests that MTALTND4 has a negligible impact on gene expression.

• RT-qPCR in MiR05 or DMEM low glucose indicates that the culture media affects gene regulation.

• This study emphasizes the importance of employing diverse approaches when examining the physiological effects on cells and the need to carefully select the appropriate culture media to interpret cellular responses and expression data accurately.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.