利用CRISPR-Cas9基因靶向在人胚胎干细胞BJNhem20中生成-肌动蛋白报告细胞系(BJNhem20 ACTB-eGFP

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-07-25 DOI:10.1016/j.scr.2025.103783

Pratiksha P. Bhat , Maneesha S. Inamdar

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引用次数: 0

摘要

-肌动蛋白是一种细胞骨架蛋白，参与多种细胞过程。在这里，我们通过CRISPR Cas9基因编辑在BJNhem20人胚胎干细胞系中产生了-肌动蛋白报告基因敲入。报告蛋白mEGFP被整合到-肌动蛋白位点，通过连接物标记蛋白的n端。报告细胞系是研究人胚胎干细胞细胞过程中-肌动蛋白动力学和实时成像跟踪细胞的重要工具。

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Generation of beta actin reporter line (BJNhem20 ACTB-eGFP) in human embryonic stem cells BJNhem20 using CRISPR-Cas9 gene targeting

Beta actin is a cytoskeletal protein that contributes to a wide range of cellular processes. Here we generated beta actin reporter knock-in in BJNhem20 human embryonic stem cell line by CRISPR Cas9 gene editing. The reporter mEGFP is integrated at the beta actin locus, tagging the N-terminal of the protein via a linker. The reporter line is a valuable tool to study beta actin dynamics during cellular process in human embryonic stem cells and to track cells by live imaging.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.