临床前小鼠脑转移瘤模型的生成、定量和表型分析。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-07-23 DOI:10.1016/j.xpro.2025.103978

Francisco Javier Rodriguez-Baena, Berta Sanchez-Laorden

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引用次数: 0

摘要

脑转移领域正在迅速扩大，但对最可靠的量化方法尚未达成共识。我们提出了一种评估小鼠颈动脉内注射肿瘤细胞后转移负荷的方案。我们描述了外科手术的步骤，大脑处理，冷冻切片和幻灯片准备，随后是表型表征。我们详细的程序量化脑转移区使用自动显微镜和半监督图像分析。有关本协议使用和执行的完整细节，请参见Rodriguez-Baena等人1。

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Protocol for generation, quantification, and phenotyping of brain metastases in preclinical mouse models.

The field of brain metastasis is rapidly expanding, yet no consensus exists on the most reliable quantification approach. We present a protocol for assessing metastatic burden in mice following intracarotid injection of tumor cells. We describe steps for surgical procedures, brain processing, cryo-sectioning, and slide preparation, followed by phenotypic characterization. We detail procedures for quantifying brain metastatic area using automated microscopy and semi-supervised image analysis. For complete details on the use and execution of this protocol, please refer to Rodriguez-Baena et al.¹.

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