从视网膜母细胞瘤患者外周血单个核细胞中提取的人诱导多能干细胞

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-07-11 DOI:10.1016/j.scr.2025.103772

Xiaoqiong Xiang, Xunda Ji

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引用次数: 0

摘要

视网膜母细胞瘤（RB）是一种常见的眼内恶性肿瘤，主要归因于肿瘤抑制基因RB1的变异。在这项研究中，我们成功地从患有遗传性RB的婴儿的外周血单个核细胞中获得了人类诱导多能干细胞（iPSC）系。生成的iPSCs表现出关键多能性标记的表达，并且没有支原体污染。此外，它们具有正常的雄性核型，并在体外分化为所有三种胚层的能力。

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Human induced pluripotent stem cells derived from peripheral blood mononuclear cells of a retinoblastoma patient

Retinoblastoma (RB) is a prevalent intraocular malignancy primarily attributed to variations in the tumor suppressor gene RB1. In this study, we successfully derived a human induced pluripotent stem cell (iPSC) line from peripheral blood mononuclear cells obtained from an infant with heritable RB. The generated iPSCs exhibit expression of key pluripotency markers and are free from mycoplasma contamination. Additionally, they possess a normal male karyotype and demonstrate the ability to differentiate into all three germ layers in vitro.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.